Cycloheximide chx treatment assay
WebJan 1, 2015 · To compare the turnover of wtSlug and Slug-4SA, these stable cells were … WebOct 8, 2024 · (c, d) Increased O-GlcNAcylation of c-Myc with 30 mM glucose was confirmed using immunoprecipitation (c) and proximity ligation assay (d). (e, f) The protein (e) and mRNA (f) expression of c-Myc was detected after treatment with 10 μM Thiamet G or OSMI-4 for 24 h. (g) c-Myc protein stability was measured using CHX (a protein …
Cycloheximide chx treatment assay
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WebApr 12, 2024 · These assays showed specific ... in absence or presence of cycloheximide (CHX) ... We observed significantly higher levels of WT Spry2 protein after 6 h of CHX treatment in PKD1/2/3-silenced ... WebSep 4, 2024 · Anisomycin is another widely-used alternative (and often used as the control to make sure any cycloheximide effect isn't off-target), but make sure the cycloheximide is working first. If...
WebApr 8, 2024 · The proteasome inhibitor MG132 (S2619), protein synthesis inhibitor cycloheximide (CHX; S7418), and autophagy inhibitor 3-Methyladenine (3-MA; S2767) were purchased from Selleck Chemicals (Cambridge, UK). ... The results of clonogenic survival assays revealed that 3-MA treatment or si-ATG5 markedly reversed the … WebApr 10, 2024 · The Saccharomyces cerevisiae Agp2 is a plasma membrane protein initially reported to be an uptake transporter for L-carnitine. Agp2 was later rediscovered, together with three additional proteins, Sky1, Ptk2, and Brp1, to be involved in the uptake of the polyamine analogue bleomycin-A5, an anticancer drug. Mutants lacking either Agp2, …
WebApr 14, 2024 · G RKO cells expressing EV or Flag-CYP1B1 were treated with 100 μg/ml CHX and harvested at the indicated times. Cells were lysed and analyzed using western blot with the indicated antibodies (left). WebMay 15, 1991 · It appears that mRNA for EGF-R accumulate within the cell during protein synthesis inhibition and upon removal of CHX, gets translated into EGF-R specific protein as judged by immuno-dot assay. We did not observe the phenomenon of 'super induction' nor much of an additive effect under condition of combined DEX and CHX treatment. MeSH …
WebJul 23, 2024 · In this study, we performed a cycloheximide (CHX) chase assay to measure the half-lives of approximately 30 human lysosomal membrane proteins (LMPs) and identified RNF152 and LAPTM4A as short-lived membrane proteins. The degradation of both proteins is ubiquitin dependent.
WebCycloheximide the most common laboratory reagent used to inhibit protein synthesis. It blocks the elongation phase of eukaryotic translation. It binds the ribosome and inhibits eEF2-mediated... famel 50WebAmong the known inhibitors of eukaryotic translation is cycloheximide (CHX, 1), the most common laboratory reagent used to inhibit protein synthesis (Fig. 1). CHX has been shown to block the elongation phase of eukaryotic translation. It binds the ribosome and inhibits eEF2-mediated translocation2. hlc adalahWebApr 10, 2024 · After 4NQO treatment, the mice were given normal water until sacrifice. In the ATRA treatment group, the mice were given ATRA (5 mg kg −1) by gavage three times a week. For the in vivo metastasis assay, 1 × 10 6 cells were injected into the tail vein of 6-week-old male NOD/SCID mice purchased from Vital River (Beijing, China). The lung ... famelabWebCycloheximide is supplied as a lyophilized powder. For a 10 mg/ml stock, carefully weigh out and reconstitute 50 mg in 5 ml DMSO or EtOH. Working concentrations and length of treatments vary depending on the desired … hl burlingameWebCycloheximide is known to inhibit protein synthesis of symbiotic Chlorella of the ciliate P. … famel 76WebForegut endoderm was isolated at stage NF25 and pre-treated for 2 hours in cycloheximide (CHX) prior to further exposure in CHX with either 50ng/mL BMP4 protein or 500nM Bio and gene expression was assayed by RT-qPCR after 8 hours of total culture. The combination of 0.2% BSA + ethanol (EtOH) is vehicle control treatment. hlc annual updateWebJan 17, 2008 · We then found that activation of TLR3 signaling in cancerous cells by poly I:C made human and murine cancer cells become sensitive to protein synthesis inhibitor cycloheximide (CHX)-induced apoptosis, and … famel 75